分析测试学报2012，Vol.31Issue(11)：1352-1357,6.DOI:10.3969/j.issn.1004-4957.2012.11.002
电化学法研究多粘菌素B与磷脂酰丝氨酸的相互作用及与其他磷脂的比较
Electrochemical Analysis on the Interaction of Polymyxin B with Phosphatidylserine and Comparison to Other Phospholipids
摘要
Abstract
A stable phospholipid bilayer membrane was constructed on Au electrode surface by using a headgroup modified phospholipid and applied as a biomembrane model to investigate the interaction of an antibiotic polymyxin B with phosphatidylserine. Polymyxin B interacted with phosphatidylserine and destroyed the bilayer structure, resulting in increasing membrane permeation. The extent of interaction was dependent on polymyxin B concentration, reaction time and presence of cholesterol. The destroyed bilayer membrane could undergo self-recovery to some extent in KC1 solution and the self-recovery was dependent on recovery time and concentration of polymyxin B. In addition, the interactions between PMB and different phospholipids were compared. Headgroup, alkyl chain length and saturation of phospholipid all affected the interaction.关键词
多粘菌素B/磷脂双层膜/磷脂酰丝氨酸/循环伏安法/相互作用Key words
polymyxin B/ phospholipid bilayer membrane/ phosphatidylserine/ cyclic voltammetry/ interaction分类
化学化工引用本文复制引用
屈凌波,冉瑜,白希希,牛真真,于岚岚..电化学法研究多粘菌素B与磷脂酰丝氨酸的相互作用及与其他磷脂的比较[J].分析测试学报,2012,31(11):1352-1357,6.基金项目
国家自然科学基金(21002093) （21002093）
教育部留学回国人员科研启动基金 （）
中国博士后科学基金资助项目 （）
