分析测试学报2012，Vol.31Issue(11)：1379-1384,6.DOI:10.3969/j.issn.1004-4957.2012.11.006
高效液相色谱检测大麻中△9-四氢大麻酚的分析方法研究
Study on Determination of Δ9-Tetrahydrocannabinol in Cannabis by High Performance Liquid Chromatography
摘要
Abstract
A quantitative method was established for the determination of A -tetrahydrocannabinol in cannabis using high performance liquid chromatography. Methanol was selected as the solvent to extract tetrahydrocannabinol from cannabis plant or resin. The separation of tetrahydrocannabinol was performed on a Shimadzu ODS - SP column( 150 mm ×4. 6 mm, 5μm) using water - acetonitrile as mobile phase by gradient elution. The primary quantitative wavelength of UV detector was set at 210 nm and the auxiliary one was at 195 nm or 220 nm. Flow rate was set at 1 ml/min, column temperature was 40 ℃ and injection volume was 10 μL. Under the optimal conditions, good linearity was achieved over the range of 0. 5 - 100. 0 mg/L, with correlation coefficients (r2) more than 0. 999 99. The limit of detection(S/N ≥.3.3) was 0. 12 mg/L and the limit of quantitation(S/N≥10) was 0.40 mg/L. Using this method to detect a cannabis resin sample, the result of uncertainty evaluation on Δ9-tetrahydrocannabinol's content was 5. 32% ±0. 17% with k value of 2. The result showed that the method was rapid, sensitive and accurate, and was applicable in the quantitative determination of cannabis plant and resin.关键词
液相色谱/四氢大麻酚/定量Key words
liquid chromatography/ tetrahydrocannabinol/ quantitative分类
化学化工引用本文复制引用
翟晚枫,张春水,高利生..高效液相色谱检测大麻中△9-四氢大麻酚的分析方法研究[J].分析测试学报,2012,31(11):1379-1384,6.基金项目
公安部重点研究计划支持项目(2009ZDYJGAES024) （2009ZDYJGAES024）
