分析测试学报2012，Vol.31Issue(11)：1406-1410,5.DOI:10.3969/j.issn.1004-4957.2012.11.011
亚甲基蓝-高效液相色谱法测定卷烟主流烟气中的硫化氢
Determination of H2S in Main Stream Smoke of Cigarette by Methylene Blue-HPLC Method
刘献军 1庄亚东 1朱怀远 1朱莹1
作者信息
- 1. 江苏中烟工业有限责任公司技术研发中心,江苏南京210019
- 折叠
摘要
Abstract
An indirect method for the determination of H2S in main stream smoke of cigarette by HPLC with DAD detector was developed. The influence of concectration of zinc acetate on trapping effectiveness of H2S in main stream smoke was investigated. The optimal conditions were using 30 mmol/L zinc acetate -20 mmol/L sodium acetate as trapping reagent system, and 180 mmol/L H2SO4 -3 mmol/L N, N-dimethyl-p-phenylenediamine dihydrochloride - 10 mmol/L ammonium ferric sulfate as derivation reagent system. The derivative product methylene blue was separated on an Agilent ZORBAX Eclipse XDB - C18( 150 mm ×4. 6 mm, 3. 5 μm), using a mobile phase composed of methanol - formic acid -water (45 : 1 : 55) at a flow rate of 0. 8 mL/min. DAD wavelength was set at 665 ran. Under the above optimal conditions, the calibration curve was linear in the concentration range of 10. 65 -532. 69 μg/L H2S(0. 1 - 5. 0 mg/L for methylene blue) with a correlation coefficient of 0. 999 89. The spiked recoveries of S2-were in the range of 96% -98% with relative standard deviations(RSDs) of 2. 4% -3. 2% . The detection limit(MDL) for H2S in main stream smoke of cigarette was 0. 12 μg/cig. The method is simple, rapid and accurate, and could be applied in the determination of H2S in main stream smoke of cigarette.关键词
硫化氢/亚甲基蓝/HPLC/卷烟主流烟气Key words
H2S/ methylene blue/ HPLC/ main stream smoke of cigarette分类
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刘献军,庄亚东,朱怀远,朱莹..亚甲基蓝-高效液相色谱法测定卷烟主流烟气中的硫化氢[J].分析测试学报,2012,31(11):1406-1410,5.
