分析测试学报2012，Vol.31Issue(11)：1436-1440,5.DOI:10.3969/j.issn.1004-4957.2012.11.017
HPLC法测定杠板归中槲皮素-3-O-β-D-葡萄糖醛酸及槲皮素的含量
Quantitative Determination of Quercetin-3-O-β-D-glucuronide and Quercetin in Polygonum Perfoliatum L.by HPLC
摘要
Abstract
An high performance liquid chromatographic method for the quantitative determination of quercetin-3-O-β-D-glucuronide and quercetin in Polygonum perfoliatum L. was developed. The separation was performed on a Lichrosher 5 -C18 column(250 mm × 4. 6 mm, 5 μm) together with a C18 guard column by gradient elution using methanol - 0. 05% phosphoric acid as mobile phase at a flow rate of 1 mL/min. The detection wavelength was set at 258 nm and the colume temperature was 25 ℃. Two compounds were successfully separated in 30 min. The calibration curves were linear in the ranges of 0. 037 6-3. 920 0 mg for quercetin-3-O-β-D-glucuronide and 0. 004 2-0. 440 0 mg for quercetin. The limits of detection ( LOD ) and limits of quantitation ( LOQ ) were 2. 509, 0. 282 ng and 6. 272, 0. 702 ng, respectively. The mean recoveries of two analytes were 99% and 98% , respectively. The established method was applied in the determination of contents of both two compounds in 15 samples obtained from different regions, and the results showed that the proportion of both compounds in different samples were great different. The proposed method is simple, rapid and accurate, and can be used for the quality control of Polygonum perfoliatum L. .关键词
杠板归/槲皮素-3-O-β-D-葡萄糖醛酸/槲皮素/高效液相色谱Key words
Polygonum perfoliatum L. / quercetin-3-O-β-D-glucuronide/ quercetin/ high performance liquid chromatography分类
化学化工引用本文复制引用
范东生,赵超,陈华国,周欣,龚小见,赵杨,杨世林..HPLC法测定杠板归中槲皮素-3-O-β-D-葡萄糖醛酸及槲皮素的含量[J].分析测试学报,2012,31(11):1436-1440,5.基金项目
国家自然科学基金(81060340) （81060340）
贵州省中药现代化科技产业研究开发专项项目((黔科合社字)[2009] 5019号) （(黔科合社字）
贵州省药物分析研究科技创新人才团队(黔科合人才团队(2011)4008) （黔科合人才团队(2011）
贵州省中药现代化科技产业研究开发专项项目(黔科合ZY [2011] 3013) （黔科合ZY [2011] 3013）
