法医学杂志2012，Vol.28Issue(5)：321-326,6.DOI:10.3969/j.issn.1004-5619.2012.05.001
窒息死大鼠肝脾傅里叶变换红外光谱变化与死亡时间的关系
Relation between PMI and FTIR Spectral Changes in Asphyxiated Rat’s Liver and Spleen
摘要
Abstract
Objective Fourier transform infrared (FTIR) spectroscopy was applied to observe the postmortem degradation process in mechanical asphyxiated rat's liver and spleen for providing a new method of estimating PMI. Methods Rats were sacrificed by mechanical asphyxia and cadavers were kept at (20±2) ℃ in a control chamber. The liver and spleen were sub-sampled from the same rat at intervals of 0-15 days postmortem and the data were measured by FTIR spectrometer. The different absorbance (A ) ratios of peaks were calculated and the curve estimation analysis between absorbance ratios (x) and PMI (y) were performed to establish mathematical models by the statistical software. Results The band absorbance ratios showed increase, decrease and stable with PML The cubic model functions showed the strongest correlation coefficient. Compared with the spleen, the liver showed a higher correlation coefficient. The A 1541/A 1 396 of liver showed the highest correlation coefficient (r=0.966). After 6-7 days postmortem, band absorbance ratios showed a steady period. Conclusion FTIR spectroscopy can be a new and efficient method to estimate PMI within 7 days.关键词
法医病理学/窒息/谱学,傅里叶变换红外/肝/脾/死亡时间/大鼠Key words
forensic pathology/ asphyxia/ spectroscopy, Fourier transform infrared/ liver/ spleen/ postmortem interval/ rats分类
社会科学引用本文复制引用
黎世莹,邵煜,李正东,邹冬华,秦志强,陈忆九,黄平..窒息死大鼠肝脾傅里叶变换红外光谱变化与死亡时间的关系[J].法医学杂志,2012,28(5):321-326,6.基金项目
国家自然科学基金资助项目(81001350) （81001350）
国家自然科学基金面上项目(81273339) （81273339）
上海市自然科学基金资助项目(09ZR1432900,12DZ2271500) （09ZR1432900,12DZ2271500）
