高等学校化学学报2012，Vol.33Issue(9)：2085-2091,7.DOI:10.3969/j.issn.0251-0790.2012.09.037
蠕虫状聚离子复合型胶束的合成、组装和胶体稳定性
Synthesis, Self-assembly and Colloidal Stability in Physiological Milieu of Worm-like PICmicelles of Poly ( ethylene glycol) -b-poly (ethylenimine)/plasmid DNA
摘要
Abstract
The block copolymers of poly (ethylene glycol)-b-poly(ethylenimine) (PEG-b-PEI) with different molecular ratios between PEG and PEI were synthesized by classical ring-opening polymerization. By simply mixing PEG-b-PEI with plasmid DNA(pDNA) , worm-like polyion complex micelles (PICmicelles) were spontaneously formed in solution. Their structures and morphologies in physiological milieu were investigated and characterized by 1 H NMR, gel permeation chromatography ( GPC ) , dynamic light scattering ( DLS), atomic force microscope(AFM) and gel electrophoresis, etc. The influence of numerous parameters, including NaCl dissociation, protein absorption, anionic species substitution and enzymatic digestion, on the colloidal stabilities of such worm-like PICmicelles were evaluated and demonstrated. The results indicated that the longer cationie segment could be helpful to efficiently condense pDNA and improve the colloidal stability against salt dissociation and anionic species substitution. However, it could also result in insufficient PEG shielding effect against protein absorption and enzymatic digestion.关键词
自组装/聚离子胶束/DNA/基因输送/嵌段共聚物Key words
Self-assembly/ PICmicelle/ DNA/ Gene delivery/ Block copolymers of PEG-b-PEI分类
化学化工引用本文复制引用
张鑫淼,张佳伟,魏光耀,刘东旭,于达慧,姚卫国,董文飞..蠕虫状聚离子复合型胶束的合成、组装和胶体稳定性[J].高等学校化学学报,2012,33(9):2085-2091,7.基金项目
国家自然科学基金(批准号:91123029,61077066和50902128)资助. （批准号:91123029,61077066和50902128）
