高等学校化学学报2012，Vol.33Issue(10)：2178-2183,6.DOI:10.7503/cjcu20120086
基于渗透脱水的自动化蛋白质结晶高通量筛选芯片
Microfluidic Device for Automated High-Throughput Protein Crystallization Screening by Osmotic Dewatering
摘要
Abstract
A self-dispensing and osmosis-based microfluidic crystallization device was reported. This device automatically dispenses droplets containing protein and precipitant into an array of crystallization chambers by pre-degassing, and then gradually concentrates these droplets at different rates by osmotic dewatering through a polydimethylsiboxane(PDMS) membrane using a row of integrated dialysis channels filled with different concentration of salt solution. This concentration process drives the protein/precipitant mixture into supersaturated , thus tending toward the crystallization state. This device allows for screening a wide range of supersatura-tion in one trial for rapid optimization of protein crystallization. The feasibility of this crystallization device is demonstrated using the model proteins of lysozyme.关键词
自动进样/高通量筛选/渗透脱水/蛋白质结晶Key words
Self-dispensing/ High-throughout screening/ Osmotic dewatering/ Protein crystallization分类
化学化工引用本文复制引用
罗娅慧,李刚,陈强,赵建龙..基于渗透脱水的自动化蛋白质结晶高通量筛选芯片[J].高等学校化学学报,2012,33(10):2178-2183,6.基金项目
国家"九七三"计划项目(批准号:2011CB707505,2012CB933303)、国家自然科学基金(批准号:60906055,61006086和30900315)、中国科学院科研装备研制项目(批准号:YZ201143)和上海市自然科学基金(批准号:10ZR1436200)资助. （批准号:2011CB707505,2012CB933303）
