广东牙病防治2012，Vol.20Issue(8)：404-408,5.
糖基化终产物对人牙龈成纤维细胞2种基质金属蛋白酶表达的影响
Effects of advanced glycation end products on the expression of matrix matelloproteinases in human gingival fibroblasts
摘要
Abstract
Objective To explore the role of advanced glycation end products (AGEs) in modulating the expression of matrix matelloproteinase-1 (MMP-1) and matrix matelloproteinase-8 (MMP-8) in human gingival fibroblasts (HGF). Methods The cultured HGF cells were randomly divided into six groups: blank control group, HSA 50 μg/mL stimulation group (negative control group) and AGE-modified human serum albumin (AGE-HSA) 0.5, 5, 50 and 100 μg/mL stimulation groups. After 24, 48 and 72 hours of culturing, MMP-1 and MMP-8 protein and mRNA expression were detected by enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase chain reaction ( QPCR) method respectively. Results At 24, 48 and 72 hours, the level of MMP-1 in 100 μg/mL AGE-HSA group was significantly gradually higher than those in HSA group, blank control group and 0. 5 μg/mL AGE-HSA group (P <0.05) . The level of MMP-1 mRNA in each concentration AGE-HSA group was significantly higher than that in HSA group (P < 0. 05). There was no statistically significant difference between the level of MMP-1 in each concentration AGE-HSA group and that in HSA group (P>0. 05). MMP-8 mRNA expression in each concentration AGE-HSA group was negative. Conclusion AGEs may promote synthesis of MMP-1. AGEs may play an important role in periodontal tissue destruction by promoting extracellular collagen degradation. AGEs had no significant effect on the expression of MMP-8 in cultured HGF according to our results.关键词
晚期糖基化终末产物/人牙龈成纤维细胞/基质金属蛋白酶Key words
Advanced glycation end products/ Human gingival fibroblast/ Matrix metalloproteinases分类
口腔医学引用本文复制引用
邓雨泉,付云,李华菁..糖基化终产物对人牙龈成纤维细胞2种基质金属蛋白酶表达的影响[J].广东牙病防治,2012,20(8):404-408,5.基金项目
广东省医学科学技术研究基金(B2010119) （B2010119）
