广东牙病防治2012，Vol.20Issue(8)：415-418,4.
盐酸米诺环素对脂多糖诱导人牙周膜成纤维细胞白细胞介素-1β mRNA 表达的影响
Influence of minocycline Hydrochloride on IL-1β mRNA expression of human periodontal ligament fibroblasts induced by lipopolysaccharide
摘要
Abstract
Objective To study the influence of minocycline hydroohioride on interleukin-1β(IL-1β) mRNA expression of human periodontal ligament fibroblagte (HPDLF) induced by lipopolysacharide ( LPS). Methods HPDLF were cultured and divided into five groups; control group, high dose group, low dose group, medium dose group and model group. IL-1β mRNA of HPDLF induced by LPS was tested by real-time fluorescent quantitative PCR. Results The relative expression levels of IL-1β mRNA showed; model group > control group > (high dose group, low dose group, medium dose group), with significant differences (P<0.01) ; however, no significant difference was found among high dose group, low dose group and medium dose group (P>0.05). Conclusion Minocycline hydrochloride can inhibit IL-1β mRNA expression of HPDLF induced by LPS, which may be one of the mechanisms of minocycline hydrochloride in treatment of periodontitis.关键词
人牙周膜成纤维细胞/盐酸米诺环素/白细胞介素-1β/实时荧光定量PCR/脂多糖Key words
Human periodontal ligament fibroblasts/ Minocycline hydrochloride/ Interleukin-1β/ Real-time fluorescent quantitative PCR/ LPS分类
医药卫生引用本文复制引用
邱枫,段昌华,冷颖,赵旭东,朱志平..盐酸米诺环素对脂多糖诱导人牙周膜成纤维细胞白细胞介素-1β mRNA 表达的影响[J].广东牙病防治,2012,20(8):415-418,4.基金项目
佛山市卫生局医学科研立项课题(2012370) （2012370）
