干旱地区农业研究2012，Vol.35Issue(5)：193-199,7.
宁夏地区苦豆子内生真菌的分离与初步鉴定
Isolation and preliminary identification of endophytic fungi from Sophora alopecuroides in Ningxia
摘要
Abstract
In order to investigate the diversity of endophyteic fungi in Sophora alopecuroides in Ningxia,the endophytic fungi was isolated using tissue isolation method from the root,stem and leaf of Sophora alopecuroides in Ningxia and identified preliminarily based on morphological characteristics in this paper. 115 endophytic fungi were isolated from 875 sterilized tissues of Glycyrrhiza uralensis in Ningxia,of which 18 isolated from roots,41 isolated from stems,56 isolated from leaves,which account for 15.7% ,35.7% and 48.6% of total endophytic fungi respectively. The result of preliminary identification showed that the 113 fungi that produced spores were classified to 7 genera under 5 families,3 orders and 2 classes. 3 belongs to Penicillium,accounting for 2.6% ; 4 belongs to Periconia ,accounting for 3.6% ; 45 belongs to Alternaria ,accounting for 39.1 % ; 9 belongs to Fusarium ,accounting for 7.8 % ; 10 belongs to Aspergillus ,accounting for 8. 7 % ; 30 belongs to Dendrophoma ,accounting for 26. 1 % ; 9 belongs to Rhizopus ,accounting for 7.8%. But Alternaria and Dendrophoma were dominant. It was concluded that the entophytes in Sophora alopecuroides in Ningxia were diversity and rich,and there were some differences at quantity and species in different organs of Sophora alopecuroides in Ningxia.关键词
苦豆子/内生真菌/分离/初步鉴定Key words
Sophora alopecuroides/endophytic fungus/isolation/preliminary identification分类
生物科学引用本文复制引用
刘建利,林勤,闫兴富..宁夏地区苦豆子内生真菌的分离与初步鉴定[J].干旱地区农业研究,2012,35(5):193-199,7.基金项目
北方民族大学国家自然基金基金前期培育项目(2011QZP02) （2011QZP02）
宁夏回族自治区自然科学基金项目(NZ11139) （NZ11139）
