广东药学院学报2012，Vol.28Issue(5)：497-501,5.DOI:10.3969/j.issn.1006-8783.2012.05.008
非洲紫罗兰原生质体的制备及不同组分培养基对细胞增殖的影响
Preparation of Saintpaulia ionantha protoplast and effect of different cultures on cell growth
摘要
Abstract
Objective To investigate the preparation of Saintpaulia ionantha protoplast and effect of different cultures on its regeneration and growth. Methods Suspended cells of Saintpaulia ionantha were made by the loose callus and cultured to determine the optimal cultivating time by means of its growth curve. Protoplasts were prepared with cellulase and pectinase mixed at a ratio of 2 to 1 , incubating for 18, 21 and 24 h, respectively, to obtain the best enzymolysis time by comparing the protoplast yields. Basic mediums were added with callus extracts, roots extracts and soil lixivium, respectively, to nurture Saintpaulia ionantha's protoplasts for 16 days. Results The rate of cell growth reached the highest, and the cell activity was 93. 8 % when suspension cells were cultured for 8 days; the maximum yield of protoplast was in 21 h under enzymolysising mixed with 4 % cellulase and 2 % pectinase and its activity was 75. 2 %. Cell walls and cell divisions were observed at the 6th day in culture medium added with callus extracts. The cell growths in callus extracts medium and roots extracts medium were significantly higher than in basic medium and soil lixivium, of which the best was callus extracts. And soil extracts couldn't promote the protoplast cell growth. Conclusion Highly active protoplasts of Saintpaulia ionantha can be produced when the suspended cells gained by the loose callus were cultured for 8 days, and then were enzymolysed by 4% cellulase and 2% pectinase for 21 h. Callus extracts medium could promote protoplasts' regeneration and growth.关键词
非洲紫罗兰/愈伤组织/悬浮细胞系/原生质体/细胞增殖Key words
Saintpaulia ionantha/ callus issue/ cell suspension/ protoplast/ cell growth分类
农业科技引用本文复制引用
陈鑫,李骏,余汝媛,张扬,赖育菠,陈志宽,罗俊明,陆幸妍..非洲紫罗兰原生质体的制备及不同组分培养基对细胞增殖的影响[J].广东药学院学报,2012,28(5):497-501,5.基金项目
2011年广东药学院校级教学改革自由申报项目 （）
