广东药学院学报2012，Vol.28Issue(5)：510-514,5.DOI:10.3969/j.issn.1006-8783.2012.05.011
大孔吸附树脂纯化槟榔中总鞣质的研究
Purification of the total tannins from Areca catechu by macroporous adsorbed resin
周本宏 1刘苗苗 2张琛霞 2梁艺小 2罗毅2
作者信息
- 1. 武汉大学人民医院,湖北武汉430060
- 2. 武汉大学药学院,湖北武汉430072
- 折叠
摘要
Abstract
Objective In order to purify the total tannins from Areca catechu, the static adsorption behaviors of eight kinds of macroporous were investigated to select the optimum macroporous resin, of which the adsorption and desorption performances were examined. Methods A modified tungsten molybdophosphate-casein colorimetric method was used to determine the total tannins, and the adsorptive capacity, the adsorption ratio and eluting ratio of tannins were calculated to screen out suitable conditions,and single factors tests were performed. Results The saturated adsorption capacity of HPD-826 resin was 46.65 mg/g. The optimum adsorption conditions of macroporous resin HPD-826 were as follows; the total tannins' concentration of 4. 30 mg/mL for 3.5 BV volume of the sample, eluting with 70% ethaol at a flow rate of 2 BV/h for 3 BV. Conclusion Macroporous resin HPD-826 showed the best properties in purifying and enriching tannins from Areca catechu.关键词
槟榔/鞣质/大孔吸附树脂/纯化Key words
Areca catechu/tannins/macroporous adsorbed resin/purification分类
医药卫生引用本文复制引用
周本宏,刘苗苗,张琛霞,梁艺小,罗毅..大孔吸附树脂纯化槟榔中总鞣质的研究[J].广东药学院学报,2012,28(5):510-514,5.
