国际病理科学与临床杂志2012，Vol.32Issue(5)：369-373,5.DOI:10.3969/j.issn.1672-7347.2012.05.001
靶向微管解聚蛋白stathmin siRNA对Aβ1-42诱发PC12细胞损伤的保护作用
Protective effect of stathmin siRNA on Aβ1-42 induced injury in PC12 cells
摘要
Abstract
Objective: To determine the effect of stathmin on the injury induced by amyloid beta-protein 1-42 (A^l-42) in rat's phaeochromocytoma cells (PC 12 cells). Methods: The shRNA expressing vectors to target stathmin gene were designed and constructed. The recombinant plasmids were transfected into PC12 cells, and the stable transfectants were selected by G418 and treated by A($l-42. RT-PCR and Western blot were used to analyze the expression levels of stathmin mRNA and protein, respectively. MTT assay was performed to detect the proliferation of the stable transfectants. The changes of apoptosis were detected by flow cytometry. Results: Compared with control cells, stathmin expression was obviously decreased in PC12 cells with transfected pSilencer4.1-s plasmid.Compared with PC12 cells (63.88?.36) and PC12-parental cells (65.78?.74) treated by A01-42, the survival rate of PC12-S cells (78.57?.22) treated by Ap"l-42 significantly increased. The apoptotic rate of PC12 cells (28.31?.65) and PC12-parental cells (26.65?.64) treated by A|5l-42 was obviously higher than that in PC12-S cells (17.77+2.37) treated by Ap 1-42. Conclusion: The downregulation of stathmin expression can obviously reduce the A(?l-42-induced injury in PC 12 cells, which lays a foundation for the potential of stathmin as a therapeutic target in the treatment of Alzheimer's disease.关键词
stathmin/PC12细胞/β淀粉样蛋白/凋亡Key words
stathmin/ PC 12 cells/ amyloid beta-protein/ apoptosis引用本文复制引用
林芳,高萍,刘昕阳,和婷,张惠中..靶向微管解聚蛋白stathmin siRNA对Aβ1-42诱发PC12细胞损伤的保护作用[J].国际病理科学与临床杂志,2012,32(5):369-373,5.基金项目
国家自然科学基金(30901579). （30901579）
