广西科学2012，Vol.19Issue(4)：345-347,3.
高效液相色谱法测定广西壮药金花茶叶中的山奈素
Determination of Kaempferol in Camellia petelotii by HPLC
摘要
Abstract
A HPLC method was established to determine kaempferol in Camellia petelotii (Mer-rll) Sealy. Ultimate(XB-C18) column was used as chromatographic column; The mobile phase was methanol-0. 4%phosphoric acid solution (55: 45);The flow rate was 1. 0 ml·min-1 and the detector wavelength was set at 360 nm;The column temperature was maintained at room temperature. The calibration curve of kaempferol was in good linearity over the range of 0. 1-0. 5μg( r =0.9998). The average recovery was 96.36%, RSD =3. 07% (n =6). This method is convenient, sensitive, reliable and suitable for determination of kaempferol in Camellia petelotii (Merrll) Sealy.关键词
含量测定/山奈素/金花茶/HPLCKey words
content determination,kaempferol, Camellia petelotii, HPLC分类
化学化工引用本文复制引用
覃兰芳,赖茂祥,梁冰,屈信成,黄云峰,胡琦敏..高效液相色谱法测定广西壮药金花茶叶中的山奈素[J].广西科学,2012,19(4):345-347,3.基金项目
广西壮药质量标准研究项目(编号:MZY2010048)资助. （编号:MZY2010048）
