广西科学2012，Vol.19Issue(4)：374-376,3.
山苍子继代培养中芽增殖效果研究
Multiplication in the Successive Transfer Culture of Litsea cubeba
摘要
Abstract
The materials of Litsea cubeba from initial culture were used to successive transfer culture. The test was designed using 3 kinds of medium:modified MS+0. 5 mg/L of 6-BA + 0. 2 mg/L of NAA,modified MS+0. 8 mg/L of 6-BA +0. 2 mg/L of NAA,and modified MS + 1. 0 mg/L of 6-BA+0. 2 mg/L of NAA,2 kinds of cutting way,and for 4 generations to cultivate, in order to analyze the proliferation influence of Litsea cubeba successive transfer culture in different growth regulator, successive transfer times and cutting way. The results showed that modified MS+1. 0 mg/L of 6-BA+0. 2 mg/L of NAA was optimal medium for successive transfer culture. When the concentration of NAA was 0. 2 mg/L, the rare of proliferation increased with the increasing of 6-BA concentration,while the callus gradually reduced; when 6-BA concentration was 1. 0 mg/L,the proliferation rate reached the highest,and seedling growth effect was the best. Successive transfer to the 4th generation, Litsea cubeba proliferation rate revealed significantly higher than the previous 3 generations,and breeding seedlings grew well dynamic. The effect of inoculation with 2~3 cluster buds was better than that of single grafting species,its breeding rate can reach 3. 57 and the seedling was well-grown.关键词
山苍子/继代培养/增殖效果Key words
Litsea cubeba, successive transfer culture,multiplication分类
农业科技引用本文复制引用
周传明,吕曼芳,陈奎,周如琼,秦武明,赵彤宁..山苍子继代培养中芽增殖效果研究[J].广西科学,2012,19(4):374-376,3.基金项目
高峰林业科技园珍贵及乡土树种试验研究项目资助. （）
