南方农业学报2012，Vol.43Issue(10)：1433-1437,5.DOI:10.3969/j.issn.2095-1191.2012.10.1433
普通野生稻稻瘟病广谱抗性基因Pi-gx(t)的遗传分析和定位
Genetic analysis and molecular mapping of a rice blast resistance gene Pi-gx(t) derived from common wild rice
摘要
Abstract
The present study was conducted to explore the resistance difference between a novel rice blast resistant germplasm RB221 derived from common wild rice and some rice varieties with known blast resistant gene. The genetic analysis and molecular mapping of this rice blast resistant gene was also carefully examined in order to determine whether the gene was a new rice blast resistant gene or not. [ Method ]Eight rice blast isolations derived from all rice growth regions of Guangxi were used to examine the resistant spectrum of RB221 and 11 NILs rice with known resistant gene, and the allelism, classic inheritance, and molecular mapping of this resistant gene were analyzed. [Result]The research results indicated that the resistant spectrums of RB221 were different from the 11 NILs with known resistant gene. RB221 was tested with the broadest resistance spectrum to all 8 blast isolations. The result of allelism examination indicated that the resistant locus of RB221 was different from the loci of Pi-3, Pi-7{t) , Pi-ta, Pi-K, and Pi-b. Genetic analysis showed that the resistance of RB221 to blast isolation 03-35E was controlled by a pair dominant gene. The gene was mapped onto chromosome 2 by the method of constructing linkage map using microsatellite markers. The gene was located between the two SSR markers RM240 and RM1092 with distances of 1.3 cm and 2.2 cm respectively from the two markers. [Conclusion]The resistance gene of RB221 , named Pi-gx(t) , was a newly discovered resistance gene mapped onto chromosome 2; its gene loci were different from those known rice blast genes that have been already mapped.关键词
普通野生稻/稻瘟病/抗性基因/遗传分析/标记定位Key words
common wild rice/ rice blast/ rice blast resistance gene/ molecular mapping/ genetic analysis分类
农业科技引用本文复制引用
颜群,潘英华,秦学毅,高汉亮,李道远,冯锐,郭辉,朱汝财..普通野生稻稻瘟病广谱抗性基因Pi-gx(t)的遗传分析和定位[J].南方农业学报,2012,43(10):1433-1437,5.基金项目
广西青年科学基金项目(桂科青0447013,桂科青0832001Z) （桂科青0447013,桂科青0832001Z）
广西农业科学院科技发展基金项目(2005002) （2005002）
