南方农业学报2012，Vol.43Issue(10)：1443-1446,4.DOI:10.3969/j.issn.2095-1191.2012.10.1443
益生菌制品中植物乳杆菌ST-Ⅲ快速定性、定量检测方法的建立
Development on rapid qualitative and quantitative detection methods for Lactobacillus plantarum ST-Ⅲ in probiotics products
摘要
Abstract
In order to provide references for the reasonable use of L. plantarum ST-Ⅲ, rapid qualitative and quantitative methods for ST-Ⅲ detection in probiotics products were established through this research. [Method]The differences in the whole genome sequences between ST-Ⅲ and the other three Ijtctnhacillus plantarum strains accessed from the NCBI database were compared and the strain specificity primers of ST-III was designed accordingly in order to carry out strain-specific pCR analysis and fluorescent quantitative real-time PCR detection. The established real-time PCR method was used to detect the quantity of ST-III in probiotics products. [ Result ]The designed primers had good specificity for ST-III strain. The size of the expected specific band presented in the ST-III samples using PCR amplification was similar to that of the aiming band. The curve of established fluorescent quantitative real-time PCR was y= -3.3691gx+39.84 (R2=0.9990), which could demand the usage of real-time PCR, and its detection limit was 2.85×103 CFU/mL. By this detection method, the quantity of ST-III in probiotics products was 3.25×105, 1.28×106, and 4.55×106 CFU/mL, respectively. [ Conclusion ] The established methods for ST-III detection were rapid, accurate, and time-saving , therefore it could be used in relevant production processes.关键词
益生菌/植物乳杆菌ST-Ⅲ/PCR/Real-time PCR/定性定量检测Key words
probiotics/ Lactobacillus plantarum ST-III/ PCR/ Real-time PCR/ qualitative and quantitative detection分类
轻工纺织引用本文复制引用
陈臣,任婧,艾连中,刘振民..益生菌制品中植物乳杆菌ST-Ⅲ快速定性、定量检测方法的建立[J].南方农业学报,2012,43(10):1443-1446,4.基金项目
科技部"十二五"科技支撑计划项目(2012BAD28B08) （2012BAD28B08）
科技部"973"计划项目(2012CB723706) （2012CB723706）
科技部农业成果转化项目(2010GB2C000143) （2010GB2C000143）
