南方农业学报2012，Vol.43Issue(10)：1480-1484,5.DOI:10.3969/j.issn.2095-1191.2012.10.1480
光密度法与计数法测定3种能源微藻细胞生长的相关性及其验证
Optical density method and cell count method for determining the growth of three energy microalgae and their correlation and verification
摘要
Abstract
The present study aimed to investigate the correlation between cell number and solution optical density (OD) of three energy microalgae and to explore the possibility of using the OD method to determine the cell growth density in order to provide a simple method and scientific base to better study the growth and physiochemistry of energy microalgae.[ Method ]Phaeodactylum tricomutum,Platymonas subcordiformis,and Chlorella vulgaris were cultured in artificial seawater enriched with f/2 solution in a plant growth chamber.Cells were counted with the help of a hemacytometer under microscope,and the OD of microalgal solution at wavelength of 680 nm was measured.The regression equations were obtained by linear fitting,and the cell densities calculated by the cell count method and the OD method were compared and validated.[ Result]There was a significant positive correlation between the cell numbers and OD680 value.The regression equations were y=1405x-59.745 (R2=0.9942),y=216.94x+0.9743(R2=0.9947),and y=2170.1x+18.569 (R2=0.9927) for P.trieornutum,P.subcordiformis,and C.vulgaris,respectively.The cell densities directly counted under the microscope were close to those converted from the regression equations.[Conclusion]The OD method was tested to be quick, simple, and efficient for determining microalgal cell growth,which could be widely applied in the growth and physiochemical studies on energy microalgae.关键词
能源微藻/光密度/细胞计数/相关性Key words
energy microalgae/optical density/cell count/correlation分类
农业科技引用本文复制引用
蔡卓平,段舜山,朱红惠..光密度法与计数法测定3种能源微藻细胞生长的相关性及其验证[J].南方农业学报,2012,43(10):1480-1484,5.基金项目
NSFC-Guangdong Province Joint Key Project (U1133003) （U1133003）
Natural Science Foundation of China (41176104,31070103) （41176104,31070103）
Natural Science Foundation of Guangdong Province Key Project (10251007002000001) （10251007002000001）
