南方农业学报2012，Vol.43Issue(10)：1553-1557,5.DOI:10.3969/j.issn.2095-1191.2012.10.1553
利用双酶法制备猴头菇氨基酸营养液
Double enzymatic preparation method for amino acid nutrition liquid using Hericium erinaceus
摘要
Abstract
This experiment was conducted to optimize the processing conditions of amino acid nutrition liquid using Hericium erinaceus in order to provide references for developing amino acid intensified foods by using Hericium erinaceus as material. [Method ]The total amino acid in amino acid nutrition liquid of Hericium erinaceus was used to determine the dosage of acidic protease and cellulose, enzymolysis temperature, time and pH value, and water-to-Herici-um erinaceus ratio by using the single factor method and orthogonal tests. [ Result ] The factor order affecting the total amino acid in liquid were displayed as followed: acidic protease dose >cellulose dose water-to-Hericium erinaceus ratio, hydrolysis time, hydrolysis temperature>hydrolysis pH. The optimum processing conditions of amino acids nutrition liquid were determined as followed: cellulose dose of 1.50%, acidic protease dose of 1.00%, hydrolysis temperature of 50℃ , hydrolysis time of 60min, hydrolysis pH of 3.5, and water-to-nericium erinaceus ratio of 25:1. Compared to the results from conducting the hot-water extraction method, the total amino acid and soluble solid content increased by 73.2 and 75.0% respectively using double enzymatic preparation method. [ Conclusion ]It is feasible to develop amino acid nutrition liquid trom Hericium erinaceus using the double enzymatic preparation method, which could increase the total amino acid and soluble solid content.关键词
猴头菇/氨基酸/营养液/酸性蛋白酶/纤维素酶Key words
Hericium erinaceus/ amino acid/ nutrition liquid/ acidic protease/ cellulose分类
轻工纺织引用本文复制引用
赵广河..利用双酶法制备猴头菇氨基酸营养液[J].南方农业学报,2012,43(10):1553-1557,5.基金项目
贺州学院科研计划项目(2012PYZX09) （2012PYZX09）
