南方农业学报2012，Vol.43Issue(10)：1569-1574,6.DOI:10.3969/j.issn.2095-1191.2012.10.1569
红鳍东方鲀组织蛋白酶L基因的电子克隆及序列分析
In silico cloning and sequence analysis of cathepsin L gene in Takifugu rubripes
摘要
Abstract
This research aimed to clone the cathepsin L gene from Takifugu rubripes through in Silico cloning and to analyze the characteristics of the predicted amino acid sequence by the bioinformatics methods in order to provide bases for further studies related to the function of cathepsin L in the immunity system. [ Method ] BLASTn was processed in the database of T. rubripes using the cathepsin L nucleotide sequence of Zebrafish (Y08321.1) as a probe; the matching sequences were subsequentially extended in the 3' and 5' direction, respectively. The coding information was estimated by DNAssist. An alignment with other cathepsin L sequences from GenBank was performed by DNASTAR. [Result] The predicted cathepsin L gene was 2005 bp in length, which contained 7 exons and 6 introns. It also contained an open reading frame (ORF) of 1011 bp encoding a polypeptide of 336 amino acids. The predicted amino acid sequence contained a catalytic triad formed by Cys25, His159, Asn175, and the conserved ERWNIN, GNFD motifs, which were all characteristics of cathepsin L. Homology analysis revealed that the deduced amino acid sequence of T. rubripes cathepsin L shared high percentage of identity compared to other species viz., Oryzias latipes and carp. [Conclusion]The motifs and catalytic active sites of T. rubripes cathepsin L were rather conserved. The T. rubripes cathepsin L shared high percentage of identity with other fish cathepsin L in the amino acid level, which was also close to other fish cathepsin L in respect to evolutionary developments.关键词
红鳍东方鲀/组织蛋白酶L/生物信息学/电子克隆/序列分析Key words
Takifugu rubripes/ cathepsin L/ bioinformatics/ in silico cloning/ sequencing analysis分类
生物科学引用本文复制引用
梁静真,饶颖竹,陈蓉,邓富琳..红鳍东方鲀组织蛋白酶L基因的电子克隆及序列分析[J].南方农业学报,2012,43(10):1569-1574,6.基金项目
国家星火计划引导项目(2011GA780033,2008GA780049) （2011GA780033,2008GA780049）
广东省科技攻关项目(2011B020307008) （2011B020307008）
湛江师范学院博士专项项目(ZL0703,ZL0704) （ZL0703,ZL0704）
南海水产经济动物增养殖广东普通高校重点实验室开放项目(2011GDU026) （2011GDU026）
