工业微生物2012，Vol.42Issue(5)：24-28,5.DOI:10.3969/j.issn.1001-6678.2012.05.005
固定化重组β-葡萄糖苷酶转化大豆异黄酮的研究
Transformation of soybean isoflavone by immobilized recombinant β - glucosidase
应欢 1殷瑜 2朱丽 2康怀侠 2陈代杰1
作者信息
- 1. 华东理工大学生物工程学院,上海200237
- 2. 上海交通大学药学院,上海200240
- 折叠
摘要
Abstract
β - glucosidase was immobilized on chitosan beads by cross - linking and adsorption. The effects of pH, temperature on glycitin transformation reaction by immobilizedβ - glucosidase, the immobilized enzyme in repeated batches and its storage stability were investigated. The results showed that three glucosides of soybean isoflavone could be transformed by β - glucosidase. The optimal pH value was 6.4 and the optimal temperature was 45 ℃ for transformation of glycitin. The enzymatic activity of immobilized β - glucosidase remained more than 85% after stored at pH 6.4, 4 ℃ for 25 days. The yield of glycitein transformed by immobilized recombinant β - glucosidase kept about 85% after 10 batches.关键词
固定化/重组β -葡萄糖苷酶/转化/大豆异黄酮Key words
immobilized/ recombinant β — glucosidase/ transformation/ isoflavone引用本文复制引用
应欢,殷瑜,朱丽,康怀侠,陈代杰..固定化重组β-葡萄糖苷酶转化大豆异黄酮的研究[J].工业微生物,2012,42(5):24-28,5.
