工业微生物2012，Vol.42Issue(5)：35-39,5.DOI:10.3969/j.issn.1001-6678.2012.05.007
产γ -氨基丁酸乳酸菌的筛选及鉴定
Screening and identification of γ- amino butyric acid- producing lactic acid bacteria
黄祖耀 1薛正莲 1苏燕南 1陈涛 1赵世光1
作者信息
- 1. 安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽芜湖241000
- 折叠
摘要
Abstract
Six strains of lactic acid bacteria were initially screened from sample of pickle, and then the content of γ -aminobutyric acid ( GABA) in the GYP fermentation broth with 1% glutamate was measured by paper chromatography and spectrophotometry. After further screened, a high -yielding GABA strain named 3# was obtained. The content of GABA could reach 8. 006 g/L after 3 days fermentation. The strain of 3# was identified as Enterococcus faecalis by morphological, physiological and biochemical characteristics and 16S rDNA analysis.关键词
乳酸菌/γ-氨基丁酸/谷氨酸脱羧酶/筛选Key words
Lactobacillus/ GABA/ glutamic acid decarboxylase/ screening引用本文复制引用
黄祖耀,薛正莲,苏燕南,陈涛,赵世光..产γ -氨基丁酸乳酸菌的筛选及鉴定[J].工业微生物,2012,42(5):35-39,5.
