工业微生物2012，Vol.42Issue(5)：50-56,7.DOI:10.3969/j.issn.1001-6678.2012.05.010
烟梗为原料固态发酵生产果胶酶
Production of pectinase from tobacco stem by solid state fermentation
杨慧芳 1许赣荣 1汤朝起2
作者信息
- 1. 江南大学生物工程学院,江苏无锡214122
- 2. 上海烟草集团有限责任公司,上海200082
- 折叠
摘要
Abstract
Production of pectinase from tobacco stem by a fungus strain JXY-17 in solid state fermentation was carried out. Single factor and orthogonal experiments were adopted for the optimization of culture media. The results indicated that the influential extent of factors affecting enzyme production by the fungus strain JXY-17 in order was the ratio of the tobacco stem to water (A) > (NH4)2SO4( B) >KH2PO4(D) > Tween-80 (C). The optimum combination for enzyme production was A3B2C2D1, I. E. Ratio of tobacco stem to water was 1:1.5, (NH4)2SO4 5. 0% , Tween-80 0. 10% , KH2PO40. 03% , KH2PO4 0.2%. The optimized fermentation and recovery conditions were the following: the suitable culture medium with the inoculum size of 25 mL, nature pH, 50 g of tobacco stem in 1000 mL triangle flask, fermented at 30 ℃ for 6 days. Under the above conditions, the highest enzyme activity of pectinase reached 8171. 35U/g, a 3. 8-fold increase compared with that of the original conditions. After recovery of pectinase, the residue tobacco stem and the composition of the crude enzyme solution were analyzed. The residue of the fermented tobacco stem could be recovered as cellulose materials. The pectin was reduced by 45% compared with that of the control (tobacco stem). The recovery of the residue tobacco stem was about 50%.关键词
烟梗/固态发酵/果胶酶Key words
tobacco stem/ solid-state fermentation/ pectinase引用本文复制引用
杨慧芳,许赣荣,汤朝起..烟梗为原料固态发酵生产果胶酶[J].工业微生物,2012,42(5):50-56,7.
