华北农学报2012，Vol.27Issue(5)：8-11,4.
SeNHX1表达蛋白的亚细胞定位及其对Na+的解毒功能
SeNHX1 Protein Localization and Its Function of Na+ -detoxification
摘要
Abstract
Yeast expression vector pYES2-SeNHX1, pYES2-GFP, pYES2-SeNHX1-GFP were introduced into the nhxl mutant strain 296H. The protein localization of SeNHXl was observed by confocal laser scanning microscope and the function of Na+ -detoxification was studied by growth of 296H with different expression vector in medium containing 0. 5 mol/L NaCl. The results showed that green fluorescence dispersed in the whole cell in 296H (pYES2-GFP) ,but only appeared on the vacuolar membrane and shaped a cyclic structure in 296H( pYES2-SeN-HX1-GFP). So SeNHX1 was guessed to be localized on the vacuolar membrane. The strains of 296H( pYES2-GFP) and 296H were inhibited for growth when cultured in medium with NaCl,where the strains with SeNHXl gene grew better. The finding suggested SeNHXl gene could complement the function of nhxl mutant. Therefore,on the base of amino acid sequence analysis and hydrophobicity analysis, SeNHXl was confirmed as vacuolar Na + /H+ antiport-ers,which conferred to the salt tolerance of plants.关键词
SeNHX1基因/酵母/表达载体/GFP绿色荧光,耐盐性/亚细胞定位Key words
SeNHX1 gene/Yeast/Expression vector/GFP green fluorescence/Salt-tolerance/SeNHXl localization.分类
生物科学引用本文复制引用
杨小玲,季静,王罡..SeNHX1表达蛋白的亚细胞定位及其对Na+的解毒功能[J].华北农学报,2012,27(5):8-11,4.基金项目
国家转基因重大专项(2009ZX08003-019B （）
2008ZX08003-005 （）
2008ZX08004-001) （）
