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玉米MADS-box基因ZAG3的表达及功能分析

李浩戈 刘彦飞 钟鸣 陈丽静 张丽 林景卫 崔振海

华北农学报2012,Vol.27Issue(5):18-21,4.
华北农学报2012,Vol.27Issue(5):18-21,4.

玉米MADS-box基因ZAG3的表达及功能分析

Expression and Functional Analysis of Maize ZAG3

李浩戈 1刘彦飞 1钟鸣 1陈丽静 1张丽 1林景卫 1崔振海1

作者信息

  • 1. 沈阳农业大学生物科学技术学院,辽宁省农业生物技术重点实验室,辽宁沈阳110866
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摘要

Abstract

In this paper,bioinformatical and expression analysis were used to examine the functions of ZAG3 ,a AGL6-\ike gene in maize. Sequence analysis indicated that ZAG3 encoded a MADS-box protein with 255 amino acids. ZAG3 protein was predicted to be subcellular located in the nucleus. Furthermore, the promoter sequenced was analysised to contain some important regulatory element, including core promoter elements, meristem expression element,abscisic acid responsiveness and gibberellin-responsiveness element, etc. Noticeable, MeJA-responsive regulatory element was not found in the promoter region,whereas the expression of ZAG3 was remarkable up-regulated in the MeJA-treated tassel by using the Q-PCR technology. These results suggest that ZAG3 gene may relate to the JA-mediated sex determination process in maize tassel.

关键词

玉米/性别决定/AGL6/ZAG3/茉莉酸

Key words

Maize/Sex determination/AGL6/ZAG3 /JA.

分类

生物科学

引用本文复制引用

李浩戈,刘彦飞,钟鸣,陈丽静,张丽,林景卫,崔振海..玉米MADS-box基因ZAG3的表达及功能分析[J].华北农学报,2012,27(5):18-21,4.

基金项目

国家自然科学基金项目(31071422) (31071422)

华北农学报

OA北大核心CSCDCSTPCD

1000-7091

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