华北农学报2012，Vol.27Issue(5)：60-64,5.
中国西门塔尔牛DGAT1和DGAT2基因组织表达谱及其在脂肪组织中的表达与背膘厚的关联分析
The Tissues Profile of DGAT1 and DGAT2 and the Association Analysis of Their Expression in the Adipose with Backfat Thickness in Chinese Simmental Cattle
摘要
Abstract
The present study discussed the developmental expression characteristics of the acyl CoA diacylglyc-erol acyltransferase genes 1 and 2 ( DGAT1 and DGAT2) and their relationship to backfat thickness (BF).30 samples from 137 slaughtered Chinese Simmental cattle were divided into thick (H) and thin (L) backfat groups,and the DCAT1 and DGAT2 genes' expression characteristics and its expression levels in different individuals adipose tissue were analyzed by quantitative PCR technique. The result indicated that both the DGAT1 and DGAT2 had different expressed in the testis, kidney , intercostal muscle, heart, pancreas, backfat, shoulder muscle, liver, greater o-mental fat,gut fat, spleen , lung, rib eye muscle and lymph. The DGAT1 and DGAT2 mRNA in adipose were higher in H group than those in L group ( P < 0. 05 ) , and correlated with backfat thickness ( P < 0. 05 ) . The results indicated that DGAT1 and DGAT2 had significant relationship with backfat thickness and were taken as two candidate genes for fattening in beef cattle.关键词
二脂酰甘油酰基转移酶/荧光定量PCR/组织表达谱/背膘厚Key words
Diacylglycerol acyltransferase (DGAT)/Real-time PCR/Tissue expression profile/Backfat thickness(BF)分类
农业科技引用本文复制引用
王国富,吴慧光,孙国权,李俊雅,王景山,王玉泉,高树新..中国西门塔尔牛DGAT1和DGAT2基因组织表达谱及其在脂肪组织中的表达与背膘厚的关联分析[J].华北农学报,2012,27(5):60-64,5.基金项目
国家科技支撑计划(2008BAD2B03) （2008BAD2B03）
国家自然科学基金(30860186) （30860186）
内蒙古自治区自然科学基金(2011MS0409 （）
2012MS0403) （）
