华北农学报2012，Vol.27Issue(5)：65-71,7.
小麦转录因子基因TaWRKY46的克隆与表达分析
Cloning and Expression Profiles of TaWRKY46,a WRKY Type Transcription Factor Gene in Wheat(Triticum aestivum L.)
摘要
Abstract
One WRKY type transcription factor gene designated as TaWRKY46 was identified in a root subtrae-tive cDNA library in which the differential expressed genes responding to the low-Pi stress were enriched. Based on the eDNA sequence of TaWRKY46, this gene was cloned in wheat cultivar Shixin828. The cDNA of TaWRKY46 was 872 bp in length, with an open reading frame of 669 bp and an encoded polypeptide of 222 aa. TaWRKY46 contains a conserved WRKY motif and a C2H2 conserved domain. Phylogenetic analysis displays a high similarity between TaWRKY46 and HvWRKY34, a WRKY transcription family gene in barley , suggesting them to be possibly derived from a same ancestor. Compared with those in sufficient-P (2 mmol/L Pi) condition,the transcripts of TaWRKY46 in roots under the deficient-P (20 |x"mol/L Pi) condition were dramatically increased, suggesting that TaWRKY46 gene was involved in the response to low P stress in the plants. In the meantime, TaWRKY46 was also obviously responding to the deficiencies of nitrogen, potassium, zinc , and calcium, as well as to be markedly up-regulated by other abiotic stresses ,such as drought, salinity, and abscisic acid ( ABA) . Therefore , TaWRKY46 has potential roles in regulating plant adaptation to nutrition deficiencies and other abiotic stress,such as drought and salinity in wheat.关键词
小麦/TaWRKY46/基因克隆/表达/非生物逆境Key words
Wheat (Triticum aestivum L. ) / TaWRKY46 / Gene cloning/ Expression/ Abiotic stress分类
生物科学引用本文复制引用
丁长欢,孙昭华,李小娟,肖凯..小麦转录因子基因TaWRKY46的克隆与表达分析[J].华北农学报,2012,27(5):65-71,7.基金项目
国家重点基础研究发展计划("973"计划)前期(2007CB116209)项目 （"973"计划）
河北省自然科学基金项目(C2011204031) （C2011204031）
河北省作物生长调控实验室资助 （）
