华北农学报2012,Vol.27Issue(5):91-95,5.
Pit-1/Hinf Ⅰ基因PCR-RFLP和鲁西黄牛与利木赞杂交牛生长性状的关联性分析
Associations of PCR-RFLP in Pit-1/Hinf Ⅰ Gene with Growth Traits in Hybrid Cattle of Luxi Yellow Cattle and Limousin
摘要
Abstract
Improving the grouth level and meat production of Luxi Yellow Cattle, this paper use PCR-RFLP technology to analysis the correlation of Pit-1 gene polymorphism and growth traits of 111 hybrid cattle of Luxi Yellow Cattle and Limousin. The results show that the Pit-I-Hint I loci of 451 bp's PCR products is polymorphism after enzymes Hint I enzyme,the allele frequency of A and B were 0. 32 and 0. 68 ,it is not fit for Hardy-Weinberg balance. The rump length of AA genotype is higher than AB and BB genotype within population (P <0.01) ,it can be used one of candidate genes;The hip width of AB genotype is higher than AA and BB genotype (P <0.05) within the group; But there are no differences among 3 genotypes in the head length, forehead width , chest circumference , chest deep and wide, body length , rump length , waist angular wide , shank circumference, body height, the cross department high,the hip higher (P >0.05).关键词
Pit-1/PCR-RFLP/生长性能/利鲁杂交牛Key words
Pit-I/PCR-RFLP/Growth traits/Hybrid cattle of Lilu分类
生物科学引用本文复制引用
刘桂芬,万发春,刘桂萍,刘晓牧,逯茂洋,谭秀文,成海建,宋恩亮..Pit-1/Hinf Ⅰ基因PCR-RFLP和鲁西黄牛与利木赞杂交牛生长性状的关联性分析[J].华北农学报,2012,27(5):91-95,5.基金项目
山东省生物基因资源发掘与种质创新利用研究项目 ()
2010年山东省优秀中青年科学家科研奖励基金(BS2010SW026) (BS2010SW026)
3-羟-3-甲基辅酶A还原酶对渤海黑牛胆固醇合成的影响研究(31100890) (31100890)
山东省自主创新重大科技专项计划(2011ZHZX1A0406) (2011ZHZX1A0406)
"十二五"农村领域国家科技计划课题(2011BAD28B04-02) (2011BAD28B04-02)