华北农学报2012,Vol.27Issue(5):184-190,7.
猪源乙型脑炎病毒河南分离株的全基因组测序及进化分析
Full-length Genomes and Phylogenetic Analysis of Three Japanese Encephalitis Virus Isolates from Swine in Henan
摘要
Abstract
In order to learn more about the complete genome and molecular evolution regular of Japanese encephalitis virus (JEV) strains isolated from swine, the full-length genome sequences of three JEV isolates, namely as BSF. ZZ-1 ,BSF. ZZ-3 and CSF. XZ-2D isolated from swine were analyzed in this paper. The results show that the length of the complete genomes of BSF. ZZ-1 ,BSF. ZZ-3 and CSF. XZ-2D was 10 977,10 977,10 976 nt,respectively. There is an insert base of " G" at the 10 701 nt site in the genomes of both BSF. ZZ-1 and BSF. ZZ-3. Compared to the virulent strain SA14 ,the homologies of nucleotide and amino acid sequences of BSF. ZZ-1 ,CSF. XZ-2D and BSF. ZZ-3 are 99. 4% and 99. 0% , respectively. Compared to the isolates HW ( HW1 ) , Hwe ( HW2 ) and SH0601 from swine,the homologies of the present three isolates are all more than 98. 0% . Phylogenetic analysises with other 30 reference isolates covering all five JEV genotypes indicated that BSF. ZZ-1 ,CSF. XZ-2D and BSF. ZZ-3 located in a same evolutionary branch,belonging to the genotype HI.关键词
猪/乙脑病毒/全基因组/系统进化分析Key words
Swine/JEV/Complete genome/Phylogenetic analysis分类
生物科学引用本文复制引用
禹乐乐,滕蔓,罗俊,迟佳琦,余祖华,柴书军,梁晓晓,张改平,王爱萍..猪源乙型脑炎病毒河南分离株的全基因组测序及进化分析[J].华北农学报,2012,27(5):184-190,7.基金项目
国家"公益性"行业(农业)科研专项经费资助(201203082-5) (农业)