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猪源乙型脑炎病毒河南分离株的全基因组测序及进化分析

禹乐乐 滕蔓 罗俊 迟佳琦 余祖华 柴书军 梁晓晓 张改平 王爱萍

华北农学报2012,Vol.27Issue(5):184-190,7.
华北农学报2012,Vol.27Issue(5):184-190,7.

猪源乙型脑炎病毒河南分离株的全基因组测序及进化分析

Full-length Genomes and Phylogenetic Analysis of Three Japanese Encephalitis Virus Isolates from Swine in Henan

禹乐乐 1滕蔓 2罗俊 2迟佳琦 2余祖华 3柴书军 2梁晓晓 2张改平 3王爱萍2

作者信息

  • 1. 郑州大学生物工程系,河南郑州450001
  • 2. 河南省农业科学院农业部动物免疫学重点开放实验室,河南省动物免疫学重点实验室,河南郑州450002
  • 3. 河南科技大学动物科技学院,河南洛阳471003
  • 折叠

摘要

Abstract

In order to learn more about the complete genome and molecular evolution regular of Japanese encephalitis virus (JEV) strains isolated from swine, the full-length genome sequences of three JEV isolates, namely as BSF. ZZ-1 ,BSF. ZZ-3 and CSF. XZ-2D isolated from swine were analyzed in this paper. The results show that the length of the complete genomes of BSF. ZZ-1 ,BSF. ZZ-3 and CSF. XZ-2D was 10 977,10 977,10 976 nt,respectively. There is an insert base of " G" at the 10 701 nt site in the genomes of both BSF. ZZ-1 and BSF. ZZ-3. Compared to the virulent strain SA14 ,the homologies of nucleotide and amino acid sequences of BSF. ZZ-1 ,CSF. XZ-2D and BSF. ZZ-3 are 99. 4% and 99. 0% , respectively. Compared to the isolates HW ( HW1 ) , Hwe ( HW2 ) and SH0601 from swine,the homologies of the present three isolates are all more than 98. 0% . Phylogenetic analysises with other 30 reference isolates covering all five JEV genotypes indicated that BSF. ZZ-1 ,CSF. XZ-2D and BSF. ZZ-3 located in a same evolutionary branch,belonging to the genotype HI.

关键词

/乙脑病毒/全基因组/系统进化分析

Key words

Swine/JEV/Complete genome/Phylogenetic analysis

分类

生物科学

引用本文复制引用

禹乐乐,滕蔓,罗俊,迟佳琦,余祖华,柴书军,梁晓晓,张改平,王爱萍..猪源乙型脑炎病毒河南分离株的全基因组测序及进化分析[J].华北农学报,2012,27(5):184-190,7.

基金项目

国家"公益性"行业(农业)科研专项经费资助(201203082-5) (农业)

华北农学报

OA北大核心CSCDCSTPCD

1000-7091

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