河南农业大学学报2012，Vol.46Issue(5)：487-492,6.
小麦谷氨酰胺合成酶基因克隆与其表达特性分析
Cloning of glutamine synthetases in wheat and analysis of their expression characteristics
摘要
Abstract
In order to study the function of glutamine synthetase ( GS) in wheat, the full-length cDNA of GS1 and GS2 were cloned by RACE and RT-PCR (The accession number of GSl is HQ840647, and GS2 is JF894116). The full length cDNA of GSl is 1 494 bp and that of GSl is 1 631 bp. The sequences and protein characteristics of GSl and GS2 were analyzed with the biological software. Semi-quantitative PCR and western-blot were used to demonstrate the transcriptional and expressional levels of GS genes in wheat leaf during seedling stage, and the results showed that both levels of GSl got higher when the leaf began to senescence while both levels of GS2 kept the highest from the leaf expanding stage to the full expansion stage.关键词
小麦/谷氨酰胺合成酶/基因克隆/基因转录/基因表达Key words
wheat/ glutamine synthetase/ gene cloning/ gene transcription/ gene expression分类
农业科技引用本文复制引用
王小纯,张同勋,李高飞,程振云,刘宁,马新明..小麦谷氨酰胺合成酶基因克隆与其表达特性分析[J].河南农业大学学报,2012,46(5):487-492,6.基金项目
国家自然科学基金项目(30771266) （30771266）
河南省高校创新人才基金项目(2010HASTIT004) （2010HASTIT004）
