河南农业大学学报2012，Vol.46Issue(5)：553-557,5.
猪流感病毒的分离鉴定及生物学特性研究
Isolation, identification and biological characteristics of swine influenza virus
摘要
Abstract
In order to understand the prevalence of swine influenza in Henan Province, 63 samples of lungs from dead pigs were collected from 11 cities of Henan and the biological characteristics of swine influenza virus were analysed. The results showed that the 3 swine influenza viruses isolated were all H1N2 subtypes, named A/Swine/HeNan/ 01/2010 (H1N2), A/ Swine/HeNan/02/2010 ( H1N2 ) and A/Swine/HeNan/03/2010( H1N2) , respectively. On this basis, these isolation strains were pu-rificated by limited dilution cloning. Biological characteristics research showed: Except that A/Swine/ HeNan/03/2010( H1N2 ) could not agglutinate the pig and cattle erythroeytes, the remained two strains could agglutinate erythrocytes of chicken, sheep, Guinea pig, rabbit, mouse, pig and cattle; Hemagglutinin thermostability test results displayed A/Swine/He Nan/01/2010 ( H1N2 ) and A/ Swine/HeNan/02/ 2010(H1N2) belonged to thermstabile type, and A/ Swine/HeNan/03/2010 (H1N2) belonged to mid-thermstabile type. A/Swine/HeNan/01/2010 (H1N2) showed higher path-ogenicity to chicken embryo, of which EID50 and ELD50 were 10 -7.16 and 10-6.54, respectively, and the others were lower. All the 3 strains were sensitive to ether, chloroform and acid and showed infectivity cytopathic effect in the MDCK cell, but they could not infect Balb/c mice in this study.关键词
猪流感/分离/鉴定/生物学特性/H1N2Key words
swine influenza/ isolation/ identification/ biological characteristics/ H1N2分类
农业科技引用本文复制引用
刘琨,张云,赵保才,胡慧,黄磊,岳耀辉,马超锋,张龙现..猪流感病毒的分离鉴定及生物学特性研究[J].河南农业大学学报,2012,46(5):553-557,5.基金项目
国家科技支撑计划项目(2010BAD04B01) （2010BAD04B01）
河南省高校科研创新团队项目(012IRTSTHN005) （012IRTSTHN005）
