河南农业大学学报2012，Vol.46Issue(5)：584-588,5.
西流湖界面沉积物中溶磷细菌筛选及其溶磷能力研究
Screening and identification of several phosphate-solubilizing bacteria from the interfacial sediment of Xiliu Lake
摘要
Abstract
Using Ca3(PO4)2 as the P source, several phosphate solubilizing bacteria, as PS-1 , PS-4, PS-11 and PS-13 , were isolated and identified from the interfacial sediment of Xiliu Lake in Zhengzhou, Henan Province. The results showed that the 4 strains had high ability of solubilizing Ca3 (PO4)2 in liquid medium, and the P concentration in the solution increased to 580. 00,437. 98, 417.40 and 283.60 μg · mL-1 at 96 h, with yield percentage of soluble P as 28. 71% , 21. 61% , 20. 58% and 13. 89% respectively. The capacity to dissolve the inorganic phosphate was closely negatively correlated to pH value of the medium. Based on 16S rDNA gene analysis, strains PS-1 , PS-4 and PS-11 were identified as Pseudomonas sp. , most closely related to P. fluorescens, P. mandelii and P. putida respectively. PS-13 was identified as Aeromonas sp.关键词
界面沉积物/溶磷细菌/筛选鉴定/溶磷能力Key words
interfacial sediment/ phosphate-solubilizing bacteria/ screening/ identification/ phosphate-solubilizing capacity分类
农业科技引用本文复制引用
屈建航,王福芳,晏加照,胡元森,赵江..西流湖界面沉积物中溶磷细菌筛选及其溶磷能力研究[J].河南农业大学学报,2012,46(5):584-588,5.基金项目
国家自然科学青年基金项目(31000065) （31000065）
河南工业大学高层次人才科研基金项目(2010BS008) （2010BS008）
