华南农业大学学报2012，Vol.33Issue(4)：498-502,5.
转天蚕素AD(CAD)基因于紫花苜蓿的研究
Expressions of Cecropin AD (CAD) in Transgenic Alfalfa
摘要
Abstract
To explore the possibility of expressing the hybrid peptide CAD in the alfalfa, Medicago sativa, the cDNA sequence encoding CAD was obtained by recursive PCR(rPCR) , and fused with elements such as il sequence, Kozak sequence and KDEL sequence to improve the expression level in the transgenic plants, and cloned into the plant transformation vector pCAMBIA1390R. This recombinant plasmid was transformed into Agrobacterium tumefacien LBA4404 and CAD gene was transformed into alfalfa by A. tumefacien-mediated method. The regenerated alfalfa plants were selected on medium 20 mg/L hygro-mycin. The results of PCR and Southern blotting showed that the CAD gene was successfully inserted into alfalfa genome. Proteins extracted from transgenic alfalfa showed a certain bacteriostatic activity to Salmonella and Staphylococcus aureus.关键词
紫花苜蓿/抗菌肽/天蚕素AD/转基因Key words
Medicago sativa/antibacterial peptide/cecropin AD/transgenic分类
生物科学引用本文复制引用
刘秀明,朱海林,杜淑环,万秋,李海燕,李校堃..转天蚕素AD(CAD)基因于紫花苜蓿的研究[J].华南农业大学学报,2012,33(4):498-502,5.基金项目
吉林省科技厅科技发展计划项目(20100147,20090244) （20100147,20090244）
高等学校科技创新工程重大项目培育资金项目(70S018) （70S018）
吉林农业大学校内启动基金(201032) （201032）
温州市科技局科技计划项目(S20100043) （S20100043）
