华南农业大学学报2012,Vol.33Issue(4):544-549,6.
猪源狂犬病病毒N和G基因的序列分析
Analysis of N and G Gene of Rabies Virus Isolated from Pig
摘要
Abstract
To understand the difference of the nucleoprotein (N) gene and glycoprotein (G) gene between the strain of pig origin and other rabies viruses. The N and G genes of rabies virus isolated from the brain of a rabid pig in Guangdong Province were amplified by RT-PCR, cloned, sequenced and compared with the genes of other rabies virus. The results showed that the sequence of nucleotides and amino acids of N genes of GD-SH01 shared 84. 3% -98. 0% and 92. 5% -99. 3% similarity, and G genes of GD-SH01 shared 80. 4% -98. 2% and 87. 8% -99. 6% similarity, respectively, compared to other viruses.关键词
狂犬病病毒/核蛋白基因N/糖蛋白基因G/序列分析Key words
rabies virus/nucleoprotein gene/glycoprotein gene/sequence analysis分类
农业科技引用本文复制引用
张莹,郭霄峰,刘祥茵,罗永文,杨先峰,黄永亮,施赫赫,傅秋玲,阳佑天,吴晓薇..猪源狂犬病病毒N和G基因的序列分析[J].华南农业大学学报,2012,33(4):544-549,6.基金项目
国家自然科学基金(31172322) (31172322)
广东省省部产学研重点项目(2010A090200083) (2010A090200083)
农业公益性行业专项(201103032) (201103032)