河南农业科学2012，Vol.41Issue(6)：144-147,4.
猪繁殖与呼吸综合征病毒GP5蛋白单克隆抗体的制备与鉴定
Development and Characterization of Monoclonal Antibody against GP5 Protein of Porcine Reproduction and Respiratory Syndrome Virus
摘要
Abstract
In order to prepare monoclonal antibodies (mAbs) against porcine reproductive and respiratory syndrome virus (PPRSV),the recombination GP5 protein of PRRSV was used to immunize BALB/c mouse and the spleen cells were fused with NS0 cells. Three hybridoma cell lines producing mAbs specific to the GP5 protein,designated as 4B7,2C5 and 2E4,were obtained after identified by indirect and inhibit ELISAs followed by limiting dilution subcloning. All of the mAbs were shown to react specifically with the Marc-145 cells infected with PRRSV BJ-4 strain in indirect immunofluorescence assay (IFA) and immunoperoxidase monolayer assays (IPMA). Ig subclass of the mAbs was determined as IgGl,and the ELISA titers of cell supernatant and ascites were 1:256 and 1:51 200, respectively. Western-blot analysis showed that the mAbs were specific to the GP5 protein of PRRSV.关键词
猪繁殖与呼吸综合征病毒/GP5蛋白/单克隆抗体Key words
porcine reproduction and respiratory syndrome virus (PRRSV)/GP5 protein/monoclonal antibody分类
农业科技引用本文复制引用
王志红,张改平,郭军庆,李青梅,王寅彪,陈稳,王爱萍..猪繁殖与呼吸综合征病毒GP5蛋白单克隆抗体的制备与鉴定[J].河南农业科学,2012,41(6):144-147,4.基金项目
国家自然科学基金面上项目(31072122) （31072122）
