河南农业科学2012，Vol.41Issue(10)：94-99,6.
马铃薯疮痂病菌拮抗菌ZWQ-1的鉴定及防效测定
Identification and Control Efficiency of Antagonistic Strain ZWQ-1 against Potato Scab Pathogens
摘要
Abstract
To definite the taxonomy of an antagonistic bacteria strain ZWQ-1 against potato scab pathogens,ZWQ-1 was identified by using biological characteristics and 16S rDNA sequences in this paper. The results showed that its 16S rDNA sequence had 99% identity to Bacillus subtilis strain(JQ398853, JN399219) by phylogenetic tree of 16S rDNA sequences, and the biological characteristics were very close to those of Bacillus subtilis. So that strain ZWQ-1 was identified as B. subtilis. The anti-bacterial activities and control efficiency to potato scab disease of ZWQ-1 were determined using paper plate method and pot tests. Pot trials indicated that the strain ZWQ-1 had good control efficiency to potato scab disease, 79. 09% to the pathogen CPS-1,78.85% to SHXHZ-3,77. 44% to CPS-3,54. 68% to CPS-2 and 49.68% to NM-10. Besides, it also showed high suppression effect on other 18 kinds of plant pathogens in plates. Hence, the strain ZWQ-] is a potential biocontrol strain in plant disease control.关键词
马铃薯疮痂病/拮抗菌/枯草芽孢杆菌/16S rDNA序列/鉴定/防效Key words
potato scab disease/antagonistic bacteria/Bacillus subtilis/16S rDNA sequence/identification/control effect分类
农业科技引用本文复制引用
李勇,郭凤柳,赵伟全,刘大群..马铃薯疮痂病菌拮抗菌ZWQ-1的鉴定及防效测定[J].河南农业科学,2012,41(10):94-99,6.基金项目
国家自然科学基金项目(30700523) （30700523）
现代农业产业技术体系建设专项资金项目(CARS-10-P12) （CARS-10-P12）
