河南农业科学2012,Vol.41Issue(11):39-44,6.
浸种温度与化学诱变剂对芝麻种子发芽的影响
Effects of Steeping Temperature and Chemical Mutagen on Seeds Germination of Sesame
摘要
Abstract
In order to study the effects of steeping temperature and chemical mutagen on seeds germination of sesame,sesame seeds were pre-soaked in water under 4 ℃ and 25 ℃ for 24 h,and the impact of different concentrations and treatment time of EMS and NaN3 on the germination potential,germination rate,germination index and vigor index of sesame were studied. The results showed that the three sesame varieties pre-soaked in water under 4 ℃ could not germinate normally in the treatment of EMS for more than 4 h or beyond 10 g/L, while those pre-soaked in water under 25 ℃ could germinate normally by treatment with 10 g/L of EMS for 6 h or 15 g/L of EMS for 4 h. In the treatment of NaN3, three sesame varieties could germinate normally after pre-soaked at both of degrees. Notably, the germination rate of sesame seeds pre-soaked under 4 ℃ was less than 50. 0% by treatment with 6 mmol/L of NaN3 for 12 h,while the three sesame varieties had a germination rate above 69. 3% after pre-soaked under 25 ℃. When sesame seeds were pre-soaked in water under 4 ℃ , EMS and NaN3, in general, played better roles under the same treating combination of concentration and time duration. In addition, the sensitivity to the EMS varied greatly among different sesame varieties,while they had a close sensitivity to NaN3. In order to obtain the desirable mutations,it is recommended to select the application of 5 g/L of EMS for 8 h or 4 mmol/L of NaN3 for 12 h after the seeds pre-soaked in water under 4 ℃ for 24 h.关键词
芝麻/浸种温度/化学诱变剂/甲基磺酸乙酯/叠氮化钠/发芽指标Key words
sesame/steeping temperature/chemical mutagen/EMS/NaN3/germination index分类
农业科技引用本文复制引用
刘艳阳,梅鸿献,武轲,郑永战,张海洋..浸种温度与化学诱变剂对芝麻种子发芽的影响[J].河南农业科学,2012,41(11):39-44,6.基金项目
国家芝麻产业技术体系项目(CARS-15) (CARS-15)