河南农业科学2012，Vol.41Issue(11)：45-48,4.
不同花生品种幼叶丛生芽诱导研究
Efficient Induction of Adventitious Buds from Peanut Leaflets of Various Varieties
摘要
Abstract
In order to screen peanut genotypes with high induction efficiency of adventitious buds and to set up a high efficient regeneration system of peanut leaflets,MS and 1/2MS medium for peanut germination were compared based on the culture response of 22 different genotypes. The differences in induction frequency among various genotypes and the effects of Carb and Cef on the adventitious buds induction were also investigated using leaflets from 4 day-germinated peanut seeds as explants. It was shown that the germination rates of peanut seeds on 1/2MS medium were higher than those on MS medium. There were significant differences in bud induction rates among 22 genotypes. Moreover,the genotypes with induction rates above 90% were Hz006,Hz001 ,Hz012 and Hz037,while Hz015 had the lowest induction rate of 31. 8%. No inhibition to bud induction was detected with 500 mg/L of Carb added to the medium and the induction rate of Hz009 was increased by 13. 5 percentage points compared with that of CK. For most of the genotypes Cef had a negative effect on bud induction and the bud induction rate of Hz010 on the medium with Cef added was significantly dropped by 48. 5 percentage points. It was concluded that 1/2MS was the suitable medium for peanut seeds germination and the genotypes of Hz006, Hz001, Hz012 and Hz037 with high induction rate were recommended for peanut genetic transformation. Additionally, Carb was a better antibiotic than Cef when they were used to control the over growth of agrobacteria on leaflet explants during the agrobacteria mediated transformation.关键词
花生/品种/丛生芽诱导/抗生素Key words
peanut/variety/clumped bud induction/antibiotics分类
农业科技引用本文复制引用
苗利娟,张新友,黄冰艳,董文召,汤丰收,秦利,高伟,韩锁义..不同花生品种幼叶丛生芽诱导研究[J].河南农业科学,2012,41(11):45-48,4.基金项目
国家花生产业技术体系建设项目(CARS-14) （CARS-14）
河南省科技创新人才计划项目(104200510003) （104200510003）
河南省科技攻关重点项目(092102110044) （092102110044）
