湖南中医药大学学报2012，Vol.32Issue(10)：3-4,7,3.DOI:10.3969/j.issn.1674-070X.2012.10.001.003.03
银花泌炎灵片对产ESBLs大肠埃希菌体外抑菌活性研究
Study on antibacterial activity of Yinhua Miyan Ling Tablet to extended-spectrum β-lactamases-producing E.coli in vitro
王槐栋 1胡亚 1张晓天 1王冀邯 1黄红兰 1王放 1倪朝辉1
作者信息
- 1. 吉林大学白求恩医学院,吉林长春130021
- 折叠
摘要
Abstract
Objective To study the antibacterial effect of Yinhua Miyan Ling Tablet to ESBLs-producing E. Coli in vitro. Methods ESBLs-producing strains were screened and confirmed from 40 E. Coli clinical strains. K-B (Kirby-Bauer) diffusion method was used to detect the antibiotic resistance. The minimum inhibitory concentrations(MICs) of Yinhua Miyan Ling Tablet to ESBLs-producing Escherichia coli and Escherichia coli ATCC25922、 ATCC35218 were determined by standard plate dilution method in vitro. Results 18 ESBLs-producing strains were confirmed among 40 clinical isolates(detection rate was 45.0%).All ESBLs-producing strains were multiple resistant strains.The resistance rates to ampicillin, cefazolin, piperacillin, cefuroxime axetil and cefaclor were more than 90%. The resistance rates to imipenem, piperacillin/tazobactam, amikacin, amoxicillin/clavulanic acid, cefepime, and cefoxitin were lower, in less than 30%. The MIC of Yinhua Miyan Ling Tablet to ESBLs-producing Escherichia coli clinical stains and standard control strains ATCC25922 and ATCC35218 were all between 200mg/mL-100mg/mL. And there was no significant difference (P>0.05). Conclusion Yinhua Miyan Ling Tablet has a strong antibacterial activity to extended- spectrum P -lactamases-producing E-coli in vitro.关键词
银花泌炎灵片/β-内酰胺酶/大肠杆菌/抑菌活性/最低抑菌浓度Key words
Yinhua Miyan Ling Tablet/ β -lactamase/ E. Coli/ Antibacterial activity/ Minimum inhibitory concentration分类
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王槐栋,胡亚,张晓天,王冀邯,黄红兰,王放,倪朝辉..银花泌炎灵片对产ESBLs大肠埃希菌体外抑菌活性研究[J].湖南中医药大学学报,2012,32(10):3-4,7,3.
