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汞胁迫对菹草无菌苗生理代谢的影响

贾荣 施国新 乔绪强 田秀丽 徐君 徐小颖 杨海燕

湖泊科学2012,Vol.24Issue(6):899-904,6.
湖泊科学2012,Vol.24Issue(6):899-904,6.

汞胁迫对菹草无菌苗生理代谢的影响

Hg2+ stress on physiological metabolism in sterile seedlings of Potamogeton crispus L.

贾荣 1施国新 1乔绪强 1田秀丽 1徐君 1徐小颖 1杨海燕1

作者信息

  • 1. 南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,南京210046
  • 折叠

摘要

Abstract

Sterile seedlings of Potamogelon crispus L. Were cultured by tissue culture technique. They were exposed to 0, 5, 10, 15, 20 μmol/L Hg+ respectively for 3 days and analyzed with reference to its toxic impact on total chloroplyll content, chloroplyll autofluorescence, the ATPase activity and mineral nutrition absorption under lab conditions. Results revealed that with increasing Hg2+ concentration, the intensity of chloroplyll autofluorescence and chlorophyll decreased gradually. The content of soluble protein increased under 5 μmol/L Hg2+ stress, and then followed by a decline in the present of higher concentration of Hg2+ , which was the same as the change of Ca2+ Mg2 + -ATPase activity. Na +K + -ATPase also decreased with the rise of Hg2+ concentration. The absorption of Na, K, Ca, Mg was affected under Hg2 + stress. It was concluded that photo system and normal physiological metabolism were disordered.

关键词

菹草无菌苗/Hg2+胁迫/光合系统/ATP酶

Key words

Sterile seedlings of Potamogelon crispus L. / Hg2+ stress/ photo system/ ATPase

引用本文复制引用

贾荣,施国新,乔绪强,田秀丽,徐君,徐小颖,杨海燕..汞胁迫对菹草无菌苗生理代谢的影响[J].湖泊科学,2012,24(6):899-904,6.

基金项目

国家自然科学基金项目(30870139)资助. (30870139)

湖泊科学

OA北大核心CSCDCSTPCD

1003-5427

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