华中师范大学学报（自然科学版）2012，Vol.46Issue(5)：591-595,5.
鱇浪白鱼野生与养殖群体遗传多样性的ISSR分析
ISSR analysis on genetic diversity between the wild and cultured populations of Anabarilius grahami
摘要
Abstract
We assessed the genetic diversity and genetic structure between wild and cultured populations of this species using ISSR PCR (13 primers). Ninety-seven bands were amplified and 72 bands displayed polymorphic. The genetic diversity of the wild population was higher (PPB = 62. 89%; H=0. 249 0; 1 = 0. 363 6) than that of the cultured population (PPB = 58. 76% ; H = 0. 226 7; 7=0. 331 4). In total, the genetic diversities of both populations were in high levels. The Gst value based on Nei's genetic diversity analysis was 0. 082 6, while Fst value resulted from AMOVA was 0. 079 6. Both the two statistics revealed that there were about 8% genetic differentiation existed between the two populations, that was, most genetic differentiation occurred within the populations. UPMGA cluster showed that the individuals of the two populations were mostly assembled respectively, and then mixed each other, the Nm value (5. 55) was high. These results indicated that there were some degree of genetic differentiation between wild and cultured populations, but the genetic variation did not reach to the level of the populations, there were certain communication spaces between the two populations.关键词
鱇浪白鱼/ISSR/野生群体/养殖群体/遗传多样性Key words
Anabarilius grahami/ ISSR/ wild population/ cultured population/genetic diversity分类
生物科学引用本文复制引用
刘红艳,熊飞,董元火,张繁荣,杨东,余来宁..鱇浪白鱼野生与养殖群体遗传多样性的ISSR分析[J].华中师范大学学报（自然科学版）,2012,46(5):591-595,5.基金项目
国家自然科学基金项目(40901037,51109091) （40901037,51109091）
湖泊与环境国家重点实验室开放基金项目(2010SKL011) （2010SKL011）
武汉市教育局市属高校科学研究项目(2010060). （2010060）
