激光生物学报2012，Vol.21Issue(2)：131-135,5.DOI:10.3969/j.issn.1007-7146.2012.02.007
Folin-ciocalteu比色法测桑叶中多酚含量
Determination of the Total Content of Polyphenols in the Leaf of Mulbery by Folin-ciocalteu Colorimetry
摘要
Abstract
A quantitative method for the determination of the total content of polyphenols in the leaf of mulberry was studied by using Folin-ciocalteu colorimetry with gallic acid as standard. The results showed that the total polyphenol contents of the leaf of mulberry could be well calculated according to their colorimetric absorption at 760 nm by applying Folin-ciocalteu reagent 2. 0 mL and 20% Na2CO3 5. 0 mL at 30℃ for 2 h, and the linear range of standard curve was 10.0～100.0 mg/L. Stability, precision, repetition and recovery rate were in the range of 0. 2969% ～2. 502% by RSD. Therefore, Folin-ciocalteu colorimetry is convenient, fast, and accurate to determine total polyphenols of the leaf of mulbery.关键词
桑叶/多酚/Folin-ciocalteu比色法Key words
the leaf of mulberry/ polyphenol/ Folin-ciocalteu colorimetry分类
农业科技引用本文复制引用
张军,李江遐,陆翠珍,宛晓春,刘朝良..Folin-ciocalteu比色法测桑叶中多酚含量[J].激光生物学报,2012,21(2):131-135,5.基金项目
安徽省教育厅自然基金(KJ2008B215) （KJ2008B215）
