激光生物学报2012，Vol.21Issue(2)：136-140,5.DOI:10.3969/j.issn.1007-7146.2012.02.008
转GFP::cDNA基因拟南芥筛选及相关基因的功能分析
Screening Transgenic Arabidopsis Expressing GFP:: cDNA Fusions and Functional Analysis of Related Gene
摘要
Abstract
Transgenic Arabidopsis expressing random GFP: :cDNA fusions was used in this study. 58 Basta-resistant transgenic lines which expressing GFP: :cDNA fusions in nuclei or in both nuclei and cytoplasm were obtained. The cD-NA fragment inserted into the transgenic plants were cloned and analyzed, and only four lines which express an in-frame fusion were obtained. Phynotype of one transgenic line R2, which expressing an in-frame fusion to a Gly-rich carboxy-terminal domain of AtGRP8 protein, was analyzed. The hypocotyl of Rl seedlings were shorter than that of wild type col-4 grown in continuous white, blue or red light, especially under relative lower fluence rate white ( less than 100 μmol m-2 s-1) or blue (less than 75μmol m-2 s-1) light, but there was no difference in the hypocotyl length when grown in the dark. The results indicated that AtGRP8 may be involved in controlling the photomorphogenesis of Arabidopsis seedling through its Gly-rich carboxy-terminal domain.关键词
GFP/融合蛋白/AtGRP8/拟南芥Key words
GFP/ fusion protein/ AtGRP8/ Arabidopsis分类
生物科学引用本文复制引用
唐冬英,曾建新,赵小英,刘选明..转GFP::cDNA基因拟南芥筛选及相关基因的功能分析[J].激光生物学报,2012,21(2):136-140,5.基金项目
国家自然科学基金项目(31171176) （31171176）
湖南省青年人才培养重点联合基金(11JJA002) （11JJA002）
