激光生物学报2012，Vol.21Issue(2)：176-180,5.DOI:10.3969/j.issn.1007-7146.2012.02.016
超声波辅助农杆菌介导的小麦遗传转化初步研究
Sonication-assisted Agrobacterium-mediated Transformation of Wheat
摘要
Abstract
An improved method , sonication-assisted Agrobacterium-mediated transformation method of wheat seeds, is reported in the present paper. Germinated seeds were treated in sonicator for 20 min, and then, they subjected to transform by Agrobacterium. Subsequently, the seeds were sowed and cultivated for producing new generation seeds, under normal cultivation conditions. According to kanamycin selection experiment, all the four varieties tested showed kanamy-cin resistance. PCR results suggested that these resistant plants are transformant plants. The results of GUS staining showed the sonication-assisted method resulted in transformed cells at the base of seedlings, while no transformed cell was found in those of controls. The results indicated that it is feasible for obtaining transformed plants of wheat with genetic characteristics using sonication-assisted Agrobacterium-mediated transformation.关键词
超声波/转化/小麦/GUS染色Key words
ultrasound/ transformation/ wheat/ GUS staining分类
农业科技引用本文复制引用
王旭明,何道一,陈丽萍..超声波辅助农杆菌介导的小麦遗传转化初步研究[J].激光生物学报,2012,21(2):176-180,5.基金项目
安徽省自然科学基金项目(070411008) （070411008）
