解剖学杂志2012，Vol.35Issue(3)：327-331,369,封4,7.
磁刺激对小鼠海马原代神经元即刻早期基因和细胞骨架蛋白表达的影响
Influence of magnetic stimulation on the expression of immediate early genes and cytoskeletal protein in primary cultured mouse hippocampal neurons
摘要
Abstract
Objective:To observe the effects of magnetic stimulation on the expression of c-fos, activity regulated cytoskeletal protein, microtubule-associated protein 2 and neurite growth in primary cultured mouse hippocampal neurons, and to explore the possible mechanism of magnetic stimulation on cell neurite growth. Methods:The primary cultured hippocampal neurons were divided into five groups: control group, sham group, 20% maximum stimulus intensity group (20% intensity), 30% maximum stimulus intensity group (30% intensity), and 40% maximum stimulus intensity group (40% intensity). The neurons were stimulated at a rate of 1 Hz after 24h, and the maximum output intensity of magnetic field was 3. 7 Tesla. Continuous stimulation for 5d, the stimulation coil was held paralleled 1 cm above the dish. Cellular immunofluorescence staining was executed immediately in the fifth day after stimulation; the immunofluorescence intensity of c-fos, Arc and MAP2-positive neurons was detected, and the multiple neurites neurons and the cell neurite length of MAP2 positive neurons were counted. Meanwhile, semiquantitative RT-PCR and Western blotting were applied to verify the results. Results:The percentage of multiple neurites neurons(n≥2)and the cell neurite length in each stimulation group was significantly higher than that in the control group. Meanwhile, it was higher in the 30% intensity group than that in 20% and 40% intensity groups, and there was significant difference in the results. C-fos positive neurons proportion and the immunofluorescence intensity of Arc and MAP2 in the 30% intensity group was significantly higher than that in the related control group. The results of Western blotting and RT-PCR were consistent with the immunofluorescence. Conclusion: Magnetic stimulation can promote the neurite outgrowth of primary cultured hippocampus neuron, and the mechanism may be related to the up-regulation of c-fos, Arc and MAP2.关键词
磁刺激/海马神经元/免疫荧光/c-fos/活性调节的细胞骨架蛋白/微管相关蛋白2Key words
magnetic stimulation/ hippocampal neuron/ immunofluorescence/ c-fos/ activity regulated cytoskeletal protein/microtubule-associated protein 2引用本文复制引用
张展翅,马隽,栾峰,康林,苏玉红,王彦永,王铭维,崔慧先..磁刺激对小鼠海马原代神经元即刻早期基因和细胞骨架蛋白表达的影响[J].解剖学杂志,2012,35(3):327-331,369,封4,7.基金项目
国家973计划前期研究专项(2010CB535005) （2010CB535005）
