解剖学杂志2012，Vol.35Issue(4)：444-447,4.
胰岛素样生长因子1基因转染胚胎干细胞诱导分化的心肌细胞移植治疗心肌梗死
Implantation of myocardial cells differentiated from IGF-1 expressing embryonic stem cells to treat rat myocardial infarction
杨虹 1邓成国 1柯奇周 1郭青平1
作者信息
- 1. 湖北医药学院组织学与胚胎学教研室,十堰442000
- 折叠
摘要
Abstract
Objective:To explore the feasibility that myocardial cells induced from insulin-like growth factor 1 (IGF-1) expressing embryonic stem (ES) cells to treat rat myocardial infraction. Methods: The recombinant containing cDNA segment of human IGF-1 was packed in PA317 cells mediated by Lipofectamine 2000, and transfected into ES cells. After the expression of IGF-1 was analyzed, the ES cells were induced into myocardial cells. And then, rat acute infracted myocardium was treated by implantation of myocardial cells differentiated from IGF-1 expressing ES cells used as IGF-1 group, or myocardial cells differentiated from ES cells used as myocardial cells group, or DMEM medium used as control. Results: The myocardial cells induced from IGF-1 expressing ES cells showed upregulated expression of human IGF-1, compared to those in control. Immunohistochemical analysis indicated that implanted myocardial cells survived in infracted myocardium, and then, caused more neovasculature. Conclusion: The myocardial cells containing high expressing IGF-1 can promote the formation of neovessels, therefore, is benefit to survival of implanted cells, and improve the cardiac function.关键词
胰岛素样生长因子-1/基因/转染/心肌细胞/移植/心肌梗死Key words
insulin-like growth factor-1/ gene/ transfection/ myocardial cells/ implantation/ myocardial infraction引用本文复制引用
杨虹,邓成国,柯奇周,郭青平..胰岛素样生长因子1基因转染胚胎干细胞诱导分化的心肌细胞移植治疗心肌梗死[J].解剖学杂志,2012,35(4):444-447,4.
