解剖学杂志2012，Vol.35Issue(4)：451-454,4.
结缔组织生长因子-siRNA对糖尿病肾病大鼠肾组织中转化生长因子-β1及层黏连蛋白表达的影响
Effect of connective tissue growth factor-siRNA on expression of TGF-β1 and fibronectin in renal tissue of diabetic nephropathy rat
摘要
Abstract
Objective:To investigate the effect of CTGF-siRNA on the expression of TGF-J31 and fibronectin in the renal tissue of rat with diabetes. Methods: Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin for consecutive three days. After the development of diabetes, the rats were treated with CTGF-siRNA for every two days for three times. Four weeks after the last dose of CTGF-siRNA,the rats were killed and the kidneys were removed. Immuno-histochemistry was done to observe the protein expression of TGF-β1 in the renal tissues and RT-PCR analysis was also done to examine the mRNA expression of TGF-β1 and fibronectin in the renal cortex. In order to evaluate the renal function, 24 h urine protein was analyzed at day 7, 14, 21 and 28. Results:CTGF-siRNA significantly decreased the expression of TGF-β1 protein and mRNA in the renal tissues and also greatly reduced the fibronectin mRNA expression in the renal cortex. Additionally, there was also significant reduction of urine protein at the 28th day. Conclusion:CTGF-SiRNA can inhibit the increased expression of TGF-β1 and fibronectin in the renal tissues, and also improve renal function in diabetic nephropathy rats.关键词
结缔组织生长因子/siRNA/糖尿病肾病/转化生长因子-β1/层黏连蛋白Key words
connective tissue growth factors siRNA/ diabetic nephropathy) transforming growth factor β1/ fibronectin引用本文复制引用
赵正林,张杰,袁晓环,崔荣军,王桂云,金相赞,梁在夏,赵容杰..结缔组织生长因子-siRNA对糖尿病肾病大鼠肾组织中转化生长因子-β1及层黏连蛋白表达的影响[J].解剖学杂志,2012,35(4):451-454,4.基金项目
黑龙江省教育厅回国留学人员科研基金(1154h24) （1154h24）
