解剖学杂志2012，Vol.35Issue(4)：469-472,4.
APP/PS1双转基因阿尔茨海默病模型小鼠蓝斑去甲肾上腺素能神经元的体视学分析
Sterological analysis of noradrenergic neuron of the locus coeruleus in double transgenic APP/PS1 Alzhmeimer's disease model mice
摘要
Abstract
Objective:To investigate quantitative and morphological change of noradrenergic neuron in locus coeruleus of double transgenic APP/PS1 (APP/PS1 dtg) Alzhmeimer's disease (AD) model mice. Methods: Mice were divided into two groups:a young group (5-6 months old APP/PS1 dtg and wild type mice) and an aged group (16-17 months old APP/PS1 dtg and wild type mice). The brain cryosections were cut and stained with tyrosine hydroxylase (TH) immunohistochemistry, and unbiased stereology was used to analyze the total number of TH positive neurons and its morphological changes in the locus coeruleus. Re sults: Immunohistochemically, there was a significant 23% reduction in the total number of TH-positive neurons and qualitatively abnormal TH-positive fiber morphology and swollen body of TH-positive cells in the locus coeruleus of the old-aged APP/PS1 dtg mice compared with the old-aged wild type mice. Furthermore, in the group of APP/PS1 dtg mice, there was a significant age-related increase in mean cell volume of TH-positive neurons, and the mean cell volume of TH-positive neurons in the old-aged APP/PS1 dtg group significantly increased compared with dtg young APP/PS1 dtg type mice. Conclusion: These locus coer-uleus-related changes show resemblance to those present in the brain of AD patients at postmortem examination.关键词
APP/PS1双转基因/阿尔茨海默病/蓝斑/去甲肾上腺素能神经元/体视学Key words
double transgenic APP/PS1/ Alzhmeimer's disease/ locus coeruleus/ noradrenergic neuron/ sterology引用本文复制引用
简晓红,黎绫,罗学港,雷德亮..APP/PS1双转基因阿尔茨海默病模型小鼠蓝斑去甲肾上腺素能神经元的体视学分析[J].解剖学杂志,2012,35(4):469-472,4.基金项目
湖南卫生厅科研基金(B2011-060) （B2011-060）
湖南省教育厅科研基金(09C631) （09C631）
国家大学生创新实验(101054264) （101054264）
