解剖学杂志2012，Vol.35Issue(5)：567-570,封4,5.DOI:10.3969/j.issn.1001-1633.2012.05.004
羊膜上皮细胞胶原海绵复合体的构建及其细胞特性变化
Construction of complex of rat amniotic epithelial cells and collagen sponge and its growth and cellular characteristics
摘要
Abstract
Objective: To construct complex of amniotic epithelial cells (AECs) and collagen sponge and to investigate its cellular characteristics. Methods: Rat AECs were seeded in the scaffold of collagen sponge and in the bottle for attachment culture served as a control group. The growth and morphological changes were observed with CM-Dil labeling cell membrane, Hoechest33342 labeling nucleus, HE staining, scanning electron microscopy. Cellular viability was detected with CCK-8 kit. Cellular phenotype was examined by immunohistochemistry and real time quantitative PCR. Results: Compared with the culture in the bottle, rat AECs adhered and grew well within the scaffold, proliferated more actively. Its diameter decreased. Its cellular phenotype did not change obviously, but nestin mRNA upregulated. Conclusion-. Collagen sponge and AECs is proven to have a good biocompatibility, which could maintain previous cellular characteristics of AECs and promote AECs growth and proliferation.关键词
羊膜上皮细胞/胶原海绵/生物相容性/细胞特性Key words
amniotic epithelial cells/ collagen sponge/ biocompatibility/ cellular characteristics引用本文复制引用
何俊山,许家军,齐爱青,郭兵,曹文珞,刘芳,姬瑞娟,王德伟..羊膜上皮细胞胶原海绵复合体的构建及其细胞特性变化[J].解剖学杂志,2012,35(5):567-570,封4,5.基金项目
国家973重点基础研究发展计划(2005CB724302) （2005CB724302）
常州市卫生局重大科研项目(ZD200801) （ZD200801）
