解剖学杂志2012，Vol.35Issue(5)：614-616,3.DOI:10.3969/j.issn.1001-1633.2012.05.017
电针对脊髓损伤后神经生长抑制因子Sema3A表达的影响
Effects of electro-acupuncture on expression of nerve growth inhibiting factor Sema3A after spinal cord injury
张连双 1赵伟 1李红星 1邵庆1
作者信息
- 1. 滨州医学院组织学与胚胎学教研室,烟台 264003
- 折叠
摘要
Abstract
Objective: To explore the effect of electro-acupuncture on the expression of nerve growth inhibiting factor Se-ma3A ( Sema3A) in the injured tissues of the completely transected spinal cord,and its mechanism in the repair of spinal cord injury (SCI). Methods: Forty five adult rats were divided into a normal control group,a model group and an electro-acupuncture group ( EC group). All rats underwent complete transection between segment T9 and T10 of the spinal cord. Electro-acupuncture treatment started on the same day after operation in the EC group. The rats were sacrificed on 7 d, 14 d and 28 d after operation. The expression of Sema3A in the tissues of the injured spinal cord was determined by immunohisto-chemical staining. The immunopositive gray scale of the immunoreactivity was detected with computer image analysis technique. Results: The expression of Sema3A in the injured spinal cord was apparently increased in the model group from 7 d to 14 d after operation,compared with the normal control group. The expression of Sema3A in the injured spinal cord was remarkably increased by electro-acupuncture treatment, compared with the model group. On the 28 d, the expression of se-ma3A in all 3 groups was similar, which was near to the normal level. Conclusion: Electro-acupuncture can significantly improve the expression of Sema3A, which is an inhibitor of axon regeneration after SCI. And this effect may be one of the mechanisms by which electro-acupuncture can promote functional recovery after SCI.关键词
电针/脊髓损伤/Sema3A/大鼠Key words
electroacupuncture/ spinal cord injury/ Sema3A/ rat引用本文复制引用
张连双,赵伟,李红星,邵庆..电针对脊髓损伤后神经生长抑制因子Sema3A表达的影响[J].解剖学杂志,2012,35(5):614-616,3.
